Isomerases phosphoglucose isomerase

Glucose-phosphate isomerase (phosphoglucose isomerase, PGI) 700 kU/1 PGI suspension in ammonium sulfate solution. Dilute with 3.2 M ammonium sulfate to 700 kU/1 if required. 

We describe here the enzymological characteristics and application of isomerases, especially D-xylose (glucose) isomerase, phosphoglucose isomerase, triose phosphate isomerase, L-rhamnose isomerase, L-fucose isomerase, maleate cis-trans isomerase, and unsaturated fatty acid cis-trans isomerase. N-Acetyl-D-glucosamine 2-epimerase is not an isomerase, but for convenience we will also describe the characteristics and use of the enzyme because this section deals with sugar-metabolizing enzymes. 

Figure 6.3 Multienzymatic activity test for FSA. G3P D-glyceraldehyde-3-phosphate F6P fructose-6-phosphate PGI phosphoglucose Isomerase G6P glucose-6-phosphate GPD glu-cose-6-phosphate dehydrogenase.

PHOSPHOGLUCOSE ISOMERASE Glucose-6-phosphate isomerase, PHOSPHOGLUCOISOMERASE D-Glucose 6-phosphate synthesis, 6-PHOSPHO-/3-GLUCOSIDASE GLUCOSE-1-PHOSPHATE URIDYLYLTRANS-FERASE... 

The isomerization of D-glucose to D-fructose by way of an enediol intermediate is an important step in glycolysis, a complex process (11 steps) by which an organism converts glucose to chemical energy. The substrate is not glucose itself but its 6-phosphate ester. The enzyme that catalyzes the isomerization is called phosphoglucose isomerase. 

Read, J., Pearce, J., Li, X., Muirhead, H., Chirgwin, I, and Davies, C. (2001) The crystal structure of human phosphoglucose isomerase at 1.6A resolution implications for catalytic mechanism, cytokine activity and haemolytic anaemia. J. Mol. Biol. 309, 447-463. 

Figure 7.6. The metabolic reactions involved in the conversion of glycerol to glucose, the required precursor in the formation of sophorose. Note Reaction 1 catalyzed by triose phosphate isomerase. Reaction 2 catalyzed by aldolase. Reaction 3 catalyzed by fructose 1,6-bisphosphatase. Reaction 4 catalyzed by phosphoglucose isomerase., Reaction 6 catalyzed by glucose 6-phosphatase.

Lysine Glucose Investigation of metabolic fluxes in phosphoglucose isomerase mutants [108]... 

Kruckeberg, A. L., Neuhaus, H. E., Feil, R.. Gottlieb, L. D., and Stitt, M. 1989. Decreased-activity mutants of phosphoglucose isomerase in the cytosol and chloroplast of Clarkia xantiana Impact on mass-action ratios and fluxes to sucrose and starch and estimation of flux control coefficients and elasticity coefficients. Biochem. J. 261, 457-467. 

---