Isolator gloves

Glove material of constmction and size should also be considered when designing an isolator. There is a variety of gloves available for isolator use. Be sure to install gloves that are compatible with the materials handled in the isolator. Glove manufacturers (e.g., North Safety Products, Cranston, RI Renco Corp., Manchester, MA) can provide chemical compatibility charts to help in glove selection. A popular glove on the market for analytical use is the 15 mil Hypalon (DuPont Performance... 

Ergonomics is a major concern with using isolators. There are limited choices in such things as isolator heights and isolator gloves, and the use of this equipment over an extended period of time can cause ergonomic difficulties. Some of these issues and strategies to address them are discussed below. 

The operator wears disposable gloves and puts the hands and arms in the long rubber isolator gloves, which have inflatable gaskets for tight fitting to the isolator. Half or full suits are also a possibility. 

A substance which is either hygroscopic or easily oxidized is filled in a pressurized glove box (isolator) fitted with a gas purification system in which the atmospheric air is replaced by argon. Under these conditions the contents of water and oxygen are restricted to less than i ppm. 

A substance which is highly toxic is filled in a depressurized glove box (isolator). 

The product was isolated on a preparative scale in an inert atmosphere glove box and the product formula was determined by complete elemental analysis. Yields varied depending on the specific metal/ ligand combination, but as much as 2-3 g of isolated product were obtained in some of the systems. 

The boxes can contain e. g. P205 or be rinsed with dry gas. Handling in the isolator should be done wearing rubber gloves fixed to the isolator. Balances used in such a dry gas need some modifications to avoid electrostatic charges, which could lead to substantial errors. 

The quality of the RNA is the most important factor for the success of array analysis, and great care should be taken to ensure top quality. It is essential to work as quickly as possible during cell disruption to denature RNAses found in tissue cells before they can degrade the sample RNA. RNAses are also found on lab benches and on hands. Therefore, RNAse decontamination of the bench area used for isolation of the RNA is recommended. This is done using RNAseZap. Gloves should be changed frequently. 

Promethium—147, the isotope used commercially, is isolated from fission product wastes. The radioactive materials must be handled safely in a glove box. The metal complexes either with ethlenediaminetetraacetic acid (EDTA) or diethylenetriaminepentaacetic acid (DTPA) and is isolated by elution from Dowex 50. 

A reaction kettle equipped with mechanical stirrer was placed in a glove box filled with nitrogen and heated to 120°C for 1 hour to remove moisture. The reactor was then charged with glycolide (200 g), y-caprolactone (200 g), 0.27 ml dodecanol, 700 ml of xylene, and 1.12 ml of stannous octoate and where glycolide component was added over four portions in 2-hour intervals. The mixture was then stirred at 120°C for 90 hours and the product isolated after dissolving in chloroform and precipitated in methanol. 2... 

DMAP (40 xL, 8.0 tmol, 0.1 eq, 0.2 M in anhydrous DCE), and methanesulfonyl chloride (320 xL, 160 xmol, 2.0 eq, 0.5 M in anhydrous DCM) were added to a 10x95 mm test tube. The test tube was sealed with a test tube cap and stirred in glove box for 3 h at ambient temperature. The solvent was evaporated (SpeedVac or GeneVac, vacuum, medium heat, 16 h) and the residue was dissolved in anhydrous DMF (400 p,L). TEA (80 xL, 80.0 xmol, 1.0 eq, 1 M in anhydrous DMF) and the amine (480 xL, 240.0 xmol, 3.0 eq, 0.5 M in anhydrous DMF) were added. The test tube was sealed with a test tube cap. The reaction was heated and stirred at 80°C for 5 h. The solvent was evaporated (SpeedVac or GeneVac, vacuum, medium heat, 16 h), and the residue was dissolved in DMSO (1.340 mL). The reaction mixtures were analyzed by LCMS and the products isolated by automated mass-directed HPLC. 

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