Isolated Ileum MAGNUS Technique

During the prohling process of specific and safety pharmacological characterization of a candidate compound in the target-oriented research process the side effect potential is first estimated from the binding character- 

The following agonists and antagonists (standards) are used (concentrations in g/ml bath fluid)  

Histamine 10 6 g/ml Histamine antagonists, e.g. cimetidine, ranitidine, famotidine 

Many modifications of the Magnus technique have been described in the literature, mainly with the isolated ileum (e.g., Koelle et al. 1950). 

Okwuasaba and Cook (1980) dissected the myenteric plexus and longitudinal muscle free of the underlying circular muscle according to the method of Paton (1957), Paton and Zar (1968) and stimulated the preparation with trains of supramaximal rectangular pulses of 1.0 ms duration at a frequency of 0.2 Hz. 

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