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Isocratic analysis appropriateness

The 0.25 AtG Rule When Is Isocratic Analysis More Appropriate ... [Pg.16]

A. Quantitative Determinations. One of the three isocratic separations could also be used for a quantitative determination of the amounts of FD C Blue 1 and Red 40 present in the powdered drink mix. A spectrophotometer would be needed. After scanning to determine the best wavelength for the analysis of each dye, measurements on standard solutions could be used to construct a calibration curve. The isocratic separation could then be performed, and the entire band containing each dye could be collected and diluted accurately to an appropriate volume. With the volume of the eluted bands known, the mass of dye can be calculated. [Pg.329]

The quality of an HPLC analysis is often equated with the resolution of the peaks achieved, and as previously discussed this may be optimised by adjusting the solvent composition to obtain appropriate capacity factors for the solutes. For difficult separations, isocratic elution may be inadequate and the desired resolution may only be achieved using gradient elution... [Pg.282]

See other pages where Isocratic analysis appropriateness is mentioned: [Pg.49]    [Pg.6]    [Pg.1445]    [Pg.1301]    [Pg.1373]    [Pg.341]    [Pg.162]    [Pg.351]    [Pg.138]    [Pg.150]    [Pg.590]    [Pg.51]    [Pg.398]    [Pg.89]    [Pg.95]    [Pg.541]    [Pg.1432]    [Pg.524]    [Pg.498]    [Pg.367]    [Pg.369]    [Pg.198]    [Pg.148]    [Pg.1041]    [Pg.639]    [Pg.22]    [Pg.19]    [Pg.619]    [Pg.199]    [Pg.215]    [Pg.427]    [Pg.1360]   
See also in sourсe #XX -- [ Pg.42 ]



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