IPT genes

Plant IPT genes have been identified from Arabidopsis,401-403 petunia,404 hop,405 and rice406 and constitute a small gene family. In contrast to Agrobacterium IPT, biochemical analysis revealed that plant IPT predominantly utilizes ATP and ADP rather than AMP as a prenyl acceptor401,405,406 and DMAPP as the donor396 (Figure 14). 

In addition to ipt gene, a number of genes involved in hormone signal transduction have been indentified to influence the regenerative competence of plant cells for somatic embryogenesis and/or adventitious shoot formation (Sakamoto et al., 2006 Srinivasan et al., 2007, 2011). 

Molinier, J., Thomas, C., Brignou, M. Hahne, G. (2002). Transient expression of ipt gene enhances regeneration and transformation rates of sunflower shoot apices (Helianthus animus L.). Plant Cell Reports, 21 251-256... 

Fig. 2. Analysis of vs-ipt gene expression in response to temperature elevation. Leaves were excised from four independent transgenic petunias A-D) and incubated at 25 C or at 42 C for 2 h. Total RNA was extracted immediately thereafter and induction of the hs-// / gene analyzed by m m .r. i V...

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