IPC-ICP and Other Unusual Hyphenations

A combination of IPC and inductively coupled plasma (ICP) MS was extensively explored for the speciation of phosphorus, arsenic, selenium, cadmium, mercury, and chromium compounds [108-118] because it provides specific and sensitive element detection. Selenium IPC speciation was joined to atomic fluorescent spectrometry via an interface in which all selenium species were reduced by thiourea before conventional hydride generation [119], Coupling IPC separation of monomethyl and mercuric Hg in biotic samples by formation of their thiourea complexes with cold vapor generation and atomic fluorescence detection was successfully validated [120]. The coupling of IPC with atomic absorption spectrometry was also used for online speciation of Cr(III) and Cr(VI) [121] and arsenic compounds employing hydride generation [122]. 

In the analysis of nucleotide-activated sugars, structural information was gained via IPC-ESI-MS and IPC-NMR. The NMR spectrometer was equipped with a flow cell a stop-flow valve allowed precise parking of the peak of interest to perform the NMR scan [123]. 

Online coupling of surface-enhanced resonance Raman spectroscopy and IPC proved valuable for the identification of basic dyes [124]. Circular dichroism spectroscopy is an extraordinary technique for selective detection of compounds possessing optically active adsorption bands and was successfully coupled to IPC of steroids [125]. Table 12.4. summarizes the most important features and parameters to be compared when selecting a detection mode for an IPC application. 

Characteristics of Common Detectors (Data from manufacturers literature) 

Range of Universal Selective Very selective Very selective Very selective Universal Universal and very  

---