Iohexol analysis

Shihabi, Z.K. Hinsdale, M.E. Serum iohexol analysis by micellar electrokinetic capillary chromatogr hy. Electrophoresis 2006, 27, 2458-2463. 

X-ray analysis of crystals of iohexol and serine proteinase (pancreaticporcine elastase) reveals that three molecules of iohexol are associated with elastase, with one close to the active site (subsite SI), the second in the vicinity of in subsites S2/S3, and the third located in a pocket at the surface of the protein. The association is a result of the affinity of iohexol directed toward the hydrophobic regions of the enzyme and supports the hypothesis of the contrast medium s potent inhibition of thrombin. Another example of the contrast medium-protein interaction is between iopamidol and fibrinogen or lysozyme... 

Figure 6 Analysis of fenofibric acid (F, 42 mg I h in serum by CE, with stacking. Iohexol (I) is added as internal standard. Conditions of analysis capillary of 25cm x 50pm (ID), voltage 7kV, wavelength 280nm, sample size 13% of the capillary volume. Separation buffer as in Figure 2.

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