Iodine overlayer

As is illustrated in Sections A and B, HRP is a convenient detection system. Many lectins can be purchased directly conjugated to this and other enzymes (e.g., alkaline phosphatase), enabling a single step overlay. Alternatively, iodinated lectins are commonly used (see Fig. 2). In addition, a wide variety of biotin-lectin conjugates are also available and would be used much in the same manner as is described in Section A, i.e., a two-step procedure (see also Section D). 

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