Investigation of Enzyme Kinetics

In this chapter, some principles of the kinetics of enzymatic reactions are discussed. A more detailed description of enzyme kinetics is covered in a number of textbooks and articles[45, 101-1041, First of all, a few general definitions will be given. 

(15) is the rate equation of the reaction (also called the kinetic model ). The formulation of such a differential equation for all reacting substances is the basic step in describing the kinetics of chemical/biochemical reactions. These rate equations include concentration values of the relevant reaction partners and kinetic parameters such as the rate constant k. An investigation of enzyme kinetics includes the measurement of reaction rates, the choosing of an appropriate kinetic model and the identification of the kinetic parameters. 

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In addition to the analytical applications, there was sporadic work on the employment of flow calorimetry for the investigation of enzyme kinetics [23,24]. In 1985 Owusu et al. [25] published the first report on the use of flow microcalorimetry for the study of immobilized enzyme kinetics approaching... 

In spite of the evident advantages of flow microcalorimetry, this technique has been used in only a few experimental investigations of enzyme kinetics. Several references dealing with this subject are listed in Table 1. 

The purpose of studies on enzyme properties is to select favorable reaction conditions for the investigation of enzyme kinetics. The choice of assay conditions has to be performed very carefully, and it has to be proven that the assay conditions are as close as possible to the reactor conditions of the final process. This aspect cannot be stressed too much ... 

Houston, C.T, Taylor, W.P., Widlanski, T.S., Reilly, J.R (2000) Investigation of Enzyme Kinetics Using Quench-flow Techniques with MALDI-TOF Mass Spectrometry. Anal. Chem. 72 3311-3319. 

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