Inverting glycosidases, enzyme kinetics

Scheme 5.1 Minimal kinetic scheme for an inverting glycosidase k+i is the bimolec-ular rate constant for enzyme-substrate (ES) combination, k i is the unimolecular rate constant for loss of the substrate from the enzyme and k+2 is the first-order rate constant for the chemical step in the ES (Michaelis) complex. Product loss is assumed to be fast and isomerisations of the Michaelis complex to be either non-existent or rapid and reversible.

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