Introduction protein epitopes, using

Also in the prediction of the introduction of new epitopes by protein modification immune-tolerant transgenic mice can be used. Mice transgenic for human insulin and tissue plasminogen activator have been used to identify new epitopes in products with amino acid modifications [14,15],... 

Purification and characterization of an individual MPC can be simplified by the introduction of various forms of sequence tags and functional protein domains into the subunits of the complex. This is easily accomplished in S. cerevisiae with the use of genetic recombination. These fusions have primarily three different purposes to introduce epitopes that can be specifically detected with monoclonal antibodies, to aid in purification, and to contribute a new function. 

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