Introduction - Need for LC-NMR

An initially pursued strategy consisting of an acetonitrile extraction, followed by a classical column chromatographic work-up, and subsequential comprehensive off-line NMR structural elucidation, proved to be unsuitable for two reasons on the one hand, mostly sub-fractions of asterosaponins were 

In this context, LC-NMR and LC-NMR-MS proved to be powerful tools for speeding up the process of screening fractions of biologically active natural products for novel compounds, justifying the laborious isolation and comprehensive off-line NMR examination. 

1H NMR data MS data, number of exchangeable protons 2D1H NMR data, correlation information 

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