Intramolecular binding potential

Fig. 5.9. Effect of axial stress on intramolecular binding potential (from [191).

In order to do this basic results of quantum chemistry [1,2] are recalled in this chapter which concern the strengths of intramolecular bonds and factors influencing the binding potential such as electronic excitation or ionization. 

Attention has been drawn to the potential of phosphoric acid anhydrides of nucleoside 5 -carboxylic acids (14) as specific reagents for investigating the binding sites of enzymes. For example, (14 B = adenosine) inactivates adenylosuccinate lyase from E. coli almost completely, but has little effect on rabbit muscle AMP deaminase. The rate of hydrolysis of (14) is considerably faster than that of acetyl phosphate, suggesting intramolecular assistance by the 3 -hydroxyl group or the 3-nitrogen atom. 

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