Intracellular generation of superoxide

Fig. 3 also shows the chemiluminescence rise is delayed for about 10 sec from the fluorescence rise. This suggests the generation of superoxide anion from the THP-1 cells is originated from the rise of intracellular Cslz ion concentration. 

As mentioned in section 3.1, uni-electron reduction of oxygen generates superoxide anions [19] and an important source are the electron-transport chains [23,27], Although components of these chains pass the bulk of electrons onto the next component of the chain some appear to leak electrons to oxygen [25]. In order to assess the level of any intracellularly generated... 

Figure 2. Detection of 02- in BAEC upon treatment with CsA. Left representative experiment of superoxide detection in supernatants of BAEC measured by electron spin resonance (ESR) using the superoxide-sensitive spin trap DMPO. The superoxide generator DMNQ was used as a positive control. Right flow cytometiy detection of 02 with die probe DHE. BAEC were preincubated for 1 hour with 5 pM DHE and then incubated for 2 hours with the indicated doses of CsA. Data are represented as mean intracellular fluorescence of ethidium (oxidized fluorescent form of DHE) (n = 7). p < 0.05 vs CsA vehicle (0 pM CsA).

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