Interpretive slippage

Investigators worry deeply about the potential for interpretive slippage, where they could become oblivious to possible risks. As another investigator described "I put it down to the syndrome where if you go long enough without an accident, you almost become oblivious to the fact that you will have one" (Si6-7). This is variously discussed in... 

Unlike DNA diagnostics with clinical samples, where the amount of DNA in a volume of blood or other biological fluid can be estimated with little consequence, postpurification DNAquantification is of the utmost importance in forensic sample analysis. The target mass of DNA required for effective use of the commercially available STR kits is 1 ng. When a reduced mass of DNA is added to the PCR, stochastic effects that could include allelic dropout, may make interpretation difficult. Alternatively, an excess of DNA in the PCR (>5 ng) can result in off-scale peaks, pull-up (color bleeding usually due to off-scale peaks), increased levels of stutter (small peaks typically one repeat unit shorter than the STR allele, believed to be due to slippage of the primer or template during replication) or other artifacts in the subsequent electrophoretic separation. 

It should be noted that the effect of slippage on the response of QCM could also be interpreted by introduction of a charge-dependent effective viscosity of a microscopic interfacial layer. In this case, an increase of the... 

Rh(CO)(HP BU2)2>2(m-H)(m-P Bu2)] with P BU2 bridges. he substitution reactions of [RhCp (C0)2] with PPh3 and p"Bu3 have been studied and the results are interpreted in terms of a ring slippage mechanism which facilitates associative... 

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