Intermediate chemicals analysis group development

An in-house process and intermediate chemicals analysis group was created, for liaison with the central Research analytical chemistry and quality control organization (housed in the Development component of the Research organization). 

A procedure for the determination of diuretics of different therapeutical character high (bumetanide, ethacrynic acid, furosemide), intermediate (bendroflumethiazide, chlorthalidone, hydrochlorothiazide, xipamide) and low (acetazolamide, amiloride, spironolactone, triamterene) efficacy diuretics, and the uricosuric agent probenecid, in urine samples, illustrates a method development implying the control of pH, surfactant and modifier [23]. The greatest analytical problems in the detection of these compounds are basically their wide variety of chemical structures, functional groups and protonation constants. This implies the use of several experimental conditions for their analysis with conventional aqueous-organic mobile phases and laborious liquid-liquid or solid-liquid extraction prior to chromatographic separation. In contrast, the same micellar eluent can produce a satisfactory separation after direct injection. 

---