Metabolism, intermediary, definition

Hofman T, Lees H (1953) The biochemistry of nitrifying organisms 4. The respiration and intermediary metabolism of Nitrosomonas. Biochem J 54 579-583 Holliger C, Wohlfarth G, Diekert G (1999) Reductive dehalogenation in the energy metabolism of anaerobic bacteria. FEMS Microbiol Rev 22 383-398 Hollocher TC, Tate ME, Nicholas DJD (1981) Oxidation of ammonia by Nitrosomonas europaea definitive lsO-tracer evidence that hydroxylamine formation involves a monooxygenase. J Biol Chem 256 10834-10836... 

The demonstration that acetyl-CoA is a relatively stable thiolester as well as later reports from Lynen s laboratory showing, (a) that thiolester derivatives of saturated, unsaturated and j8-ketoacids could be differentiated from one another by differences in their ultraviolet absorption spectra, and (b) that acyl-CoA analogs (i.e. thiolesters of A-acetylthioethano-lamine and panthetheine) are catalytically active substrates for sheep liver enzymes, provided the much needed tools for more definitive studies on the role of acyl-CoA derivatives in intermediary metabolism. These studies were facilitated also by the eventual development of reliable methods for the isolation of substrate quantities of CoA of relatively high purity.8... 

---