Interacting Subunits and Dissection of Interaction Domains

Characterization of Interacting Subunits and Dissection OF Interaction Domains 

Identification of a complex involving several polypeptides raises several questions What are the subunits in direct contact What is the stoichiometry of the interaction What is the nature of the fragment of these proteins mediating the contacts  

Answering these questions may provide some tools for understanding the biological function of the identified interactions, e.g., through the construction of dominant-negative mutants. Again, the various 

I thank Fabienne Mauxion, Erwin van Dijk, and Frederic Gabriel for critically reading the manuscript. This work was supported by the Fondation pour la Recherche Medicale and an 

Bateman, A., and Birney, E. (2000). Searching databases to find protein domain organization. Tit . Protein Chem. 54, 137-157. 

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