Integration of S and P into the elemental composition

On the other hand, phosphorus is bound as phosphate in macromolecules and is exclusively found in ash, which has a degree of reduction of 0. The oxygen bound to P has not been taken into account in the estimation of/o since it is found in ash. Hence, if one wants to include P in the elemental formula, one has to add 2.5 O per P included. This is illustrated in Table 8 where the elemental 

Element unit M thermoautotrophicum K. marxianus Detection limit 

Superscript indicates that the element was not present in the medium. It can be provided by yeast extract (for K. marxianus) or through leaching of steel parts of the reactor. Most of the concerned values are close to detection limit. 

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