Avian sarcoma virus integrase

Crystal structure of avian sarcoma virus integrase with bound essential eations... 

Bujacz, G., Jaskolski, M., Alexandratos, J., Wlodawer, A., Merkel, G., Katz, R. A., and Skalka, A. M. (1996). The catalytic domain of avian sarcoma virus integrase conformation of the active-site residues in the presence of divalent cations. Structure 4, 89-96. Kulkosky, J., Katz, R. A., Merkel, G., and Skalka, A. M. (1995). Activities and substrate specificity of the evolutionarily conserved central domain of retroviral integrase. Virology 206, 448-456,... 

Figure 14. Mode of action of avian sarcoma virus integrase (courtesy R.A. Katz). The viral strands are drawn with thin lines, the host DNA is drawn with heavy lines. This diagram is highly simplified in that there is another insertion site on the other strand 6 base pairs away. The resultant nucleic acid is repaired after the action of integrase.

Figure 15. Avian sarcoma virus (ASV) integrase. (a) Manganese ions in the active site of ASV integrase. Note that there are two examples of the metal ion-water-carboxylate motif in the coordination octahedron, (b) Zinc ions in the active site of ASV integrase. The two zinc ions are separated by water and the carboxylate group of Asp64.

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