Big Chemical Encyclopedia

Chemical substances, components, reactions, process design ...

Insufficient or No Response to Ca

Check the amount of free calcium in the buffers, if possible by the use of a Ca electrode. [Pg.228]

Reduce preincubation time to minimize loss of cytosolic proteins. Test the response of cells directly after SLO permeabilization. In contrast to Ca, the response to GTP[S] should not be altered by rundown (Kiraly-Borri efal., 1996). [Pg.228]

Supernatants have only been partially aspirated leading to dilution of incubation buffers and contamination with insulin secreted during [Pg.228]

See other pages where Insufficient or No Response to Ca is mentioned: [Pg.228]