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Insufficient or No Response to Ca

Check the amount of free calcium in the buffers, if possible by the use of a Ca electrode. [Pg.228]

Reduce preincubation time to minimize loss of cytosolic proteins. Test the response of cells directly after SLO permeabilization. In contrast to Ca, the response to GTP[S] should not be altered by rundown (Kiraly-Borri efal., 1996). [Pg.228]

Supernatants have only been partially aspirated leading to dilution of incubation buffers and contamination with insulin secreted during [Pg.228]


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