Inosinate formation from guanylate

These and other observations pointed to the direct formation of inosi-nate from a derivative of guanine, and an enzyme was discovered which catalyzes the irreversible reductive deamination of guanylate to inosinate. 

C. Formation of Adenylic and Guanylic Acids from Inosinic Acid. 409... 

In studies that are still preliminary, after incubation of human erythrocytes with inosine, in addition to the formation of a large quantity of IMP, new small peaks appeared on HPLC profiles with the retention times of IDP and ITP. If these peaks are, in fact, IDP and ITP, their formation will be consistent with the recent observation that human erythrocytic guanylate kinase, in contrast to the enzyme from other tissues, has weak substrate activity with IMP (Agarwal et al., 1971 Agarwal and Parks, 1972). 

The kinetics of water replacement by nucleosides such as inosine or 1-methylinosine have been recorded for cw-[Pt(OH2)2(NH3)2], [Pt(OH2)(dien)], and related complexes (dien is 1,4,7-triazaheptane). The reactions of [Pt(OH2)(i ,R-dach)] with r(GpA) [R,R-dach = 1R,2R-cyclohexanediamine r(GpA)= guanyl(3 -5 )adenosine] under pseudo-first-order conditions revealed formation of a 1 1 aqua intermediate which decayed into a variety of chelate products, in some cases reversibly. The displacement of guanosine from cw-[Pt(Gua)(NH3)2] by CN was followed by... 

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