Inoculum levels, sensitivity

In this example, the caterpillars were infected with the virus by placing 105 MC-PIBs on the surface of their diet. If this is raised to 10 MC-PIBs, the persistence of the recombinant is extended out at least one more generation. This sensitivity to inoculum levels is probably due to the requirement for a cell to be coincidentally infected with both a wild type and a recombinant virus to obtain an MC-PIB. The likelihood of this occurring should be sensitive to the initial number of viruses introduced into the caterpillars. 

Grendahl and Sung [96] tested a simple, reliable, and inexpensive assay method for the quantitation of serum levels of miconazole and other imidazole drugs. This assay, which is similar to Kirby-Bauer test, was sensitive to 0.2 pg of drug/mL and linear from <0.2 to 10 pg/mL. Concentration of inoculum and agar depth in test plates was not as critical as the type of medium, amount of inoculum or type of drug used. 

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