Injection and Detection in HPLC

The longer the time spent in any part of the We now consider the hardware required to inject sample and solvent onto the column and 

An ideal detector of any type (Table 25-3) is sensitive to low concentrations of every analyte, provides linear response, and does not broaden the eluted peaks. It is also insensitive to changes in temperature and solvent composition. To prevent peak broadening, the detector volume should be less than 20% of the volume of the chromatographic band. Gas bubbles in the detector create noise, so back pressure may be applied to the detector to prevent bubble formation during depressurization of eluate. 

Detector Approximate limit of detection (ng) Useful with gradient  

Linear range analyte concentration range over which detector response is proportional to concentration 

Dynamic range range over which detector responds in any manner (not necessarily linearly) to changes in analyte concentration (see page 71) 

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