Inhibition in solution assay

Biosensor instruments such as Biacore (General Electric) exploit the sensitivity of a surface plasmon resonance response to the mass localised near the surface of a sensor chip. Various approaches can be used for kinases. Inhibition in solution assays involve immobilisation of a target definition compound (TDQ on the sensor surface.13,30 A buffer containing the kinase is flowed over the surface so that the protein is able to bind to the immobilised TDC, giving a signal. When test compounds are included in the buffer, they can compete for the TDC-kinase interaction, allowing estimation of Kd. 

Altschuh, D. (2013) Chemical library screening using a SPR-based inhibition in solution assay simulations and experimental validation. Analytical Chemistry, 85 (18), 8787-8795. 

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