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Information in a Sensorgram

Multiple usage of one surface greatly enhances reproducibility of results and, therefore, is especially valuable for comparative studies. [Pg.1048]

Analysis can be purely qualitative (yes or no), in order to determine the presence or absence of binding activity in a particular sample, or under selected conditions. Whole complexes can be built up on the matrix, step by step, and simultaneously viewed on the screen. The concentration of biologically active molecules can be determined by use of a standard curve. Association and dissociation kinetics as well as the affinity of an interaction are calculated by the fitting routines of the evaluation software [3]. [Pg.1048]


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