Influence of the injected sample volume

The starting time is independent of the injected sample volume. 

The maximum of the peak shifts as a function of the injected volume. 

Dispersion decreases with increasing sample volume, and also increases the peak width. 

The signal eventually saturates by effect of the central portion of the plug undergoing no further dilution by the carrier. 

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There is always a linear relationship between sample injected volume and the area of the recorded peak. Regarding the use of peak height or peak width, three main scenarios can be considered, each one associated with a different influence of the injected sample volume on the recorded peak shape. 

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