Influence of Mass Transfer on the Reaction Parameters

Influence of Mass Transfer on the Reaction Parameters External diffusion 

Mass transfer can alter the observed kinetic parameter of enzyme reactions. Hints of this are provided by non-linear Lineweaver-Burk plots (or other linearization methods), non-linear Arrhenius plots, or differing Ku values for native and immobilized enzymes. Different expressions have been developed for the description of apparent Michaelis constants under the influence of external mass transfer limitations by Homby (1968) [Eq. (5.69)], Kobayashi (1971), [Eq. (5.70)], and Schuler (1972) [Eq. (5.71)]. 

Pore diffusion leads to a reduction by half of the apparent activation energy of enzyme reactions, as in the case of chemical reactions (Levenspiel, 1999). In the literature it has been reported that pore diffusion leads to enhanced apparent temperature and pH stability owing to broadening of the optimal activity regions as a function of temperature and pH activity (Karanth, 1978). Hindered pore diffusion 

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