INDEX of sucrose

Equations have been developed that determine the relationship of the refractive index of sucrose solutions between 0—85% concentration, 18—40°C, and 546—589 nm. 

Spin the samples with 200 000 x g at 4 °C for 15 -17 h. After the run, displace the gradient using a more dense solution, e.g., 40% sucrose in Soln. A, colored by a droplet Amido Black 10 B solution. The principle of a displacement apparatus is shown in Fig. 5.4. The RNA content of the fractions is measured either by reading the UV absorption at 260 nm or, if labeled material was used, by counting the radioactivity. To monitor the sucrose gradient, estimate the refractive index of the obtained fractions (concentration, density and refractive index of sucrose solutions are given in Table 8.17). 

International Scale (1936) of Refractive Indexes of Sucrose Solutions at 20°C... 

---

See also in sourсe #XX -- [ ]

---