In Vitro Selections on Complex Antigens

2 Phage Display for Target Identification 10.3.2.1 In Vitro Selections on Complex Antigens 

As an alternative to selections on cells, other antigen sources, which better maintain the in vivo antigen expression profile and that allow appropriate in vitro selection procedures, are available. Selection on tissue cryosections may result in antibodies directed to intra- and extracellular antigens on any cell type present in the tissue section, as well as antibodies binding to matrix components [77]. 

Model selections, in which phage antibodies with defined specificity are mixed with nonspecific phage and the enrichment and yield for a selection procedure is measured, provides a rapid experimental approach for studying such complex selection procedures. In order to compare different selection approaches on complex preparations, and to determine the best selection parameters for each approach, an extensive study of various in vitro and in vivo model selections was recently carried out by our group [73]. 

10 Phage Display Technology for Target Discovery in Drug Delivery Research 

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