In-Series Detection of Disulfides

Column 3 i.m ODS-Hypersil 100 x 4.6 mm Eluent 100 mmol L mono-chloroacetic acid (pH 3)-methanol (98 + 2) Flow-rate 1 mL min Detection electrode Au, +0.7 V vs. Ag/AgCl Reducing electrode Hg/Au, —0.9 V vs Ag/AgCl. 

Many people now use commercially available dual cells, which employ independent controllers, for disulfide measurement. The reductive electrode does 

Sun et have described an amalgam cell which contained three gold electrodes and which was employed to monitor the thiol and disulfide composition of complex peptide mixtures when some peptides contained both moieties. The gold electrodes were mounted in an L-shape, such that one electrode only measured thiols whilst the other two electrodes formed a reduction/detection pair for the measurement of disulfides. 

Kleinman and Richie used the in-series approach with gold amalgam electrodes to measure 12 thiols and disulfides, including GSH, GSSG, cysteine, cystine, homocysteine, homocystine and several mixed disulfides in rat tissues. The first (upstream) electrode was maintained at —1.0 V and the second (downstream) 

GSSG Citrus leaves Whole blood RPLC 5.7 pmol 25 

---