Immunolabeling protein

GSD microscopy has been shown to image immunolabeled protein clusters on the plasma membrane of hxed cells with Ar = 50-90 nm resolution using a CW laser power of a few kilowatt per square centimeter [105]. However, plain GSD microscopy is currently challenged by the fact that the repeated population of the triplet state or similar dark states augments photobleaching [105]. Nonetheless, this early concept was important for the development of far-held optical nanoscopy for a number of reasons. First, it highlighted... 

Nicolas, M.-T., Morse, D., Bassot, J. M., and Hastings, J. W. (1991). Colocalization of luciferin-binding protein and luciferase to the scintillons of Gonyaulax polyedra revealed by double immunolabeling after fast-freeze fixation. Protoplasma 160 159-166. 

Ferritin is a globular protein complex consisting of 24 protein subunits and is the main intracellular iron storage protein in both prokaryotes and eukaryotes. Ferritin is used for immunolabeling at the electron microscope level because of its distinctive shape of the crystals and their electron density. 

Sukhanova A et al (2004) Biocompatible fluorescent nanocrystals for immunolabeling of membrane proteins and cells. Anal Biochem 324 60-67... 

Roth, J. (1984) The protein A-gold technique for antigen localization in tissue sections by light and electron microscopy, in Immunolabelling for Electron Microscopy (Polak, J. M. and Vamdell, I. M., eds.), Elsevier, New York, pp. 113-122. 

Garcia-Sevilla, J.A., Ventayol, P., Busquets, X., La Harpe, R., Walzer, C., and Guimon, J. (1997) Regulation of immunolabelled mu-opioid receptors and protein kinase C-alpha and zeta isoforms in the frontal cortex of human opiate addicts. Neurosci Lett 226 29-32. 

Brorson, S.-H. 1998a. The combination of high-accelerator epoxy resin and antigen retrieval to obtain more intense immunolabeling on epoxy sections than on LR-white sections for large proteins. Micron 29 89-95. 

Josephsen, K., Smith, C. E., and Nanci, A. 1999. Selective but nonspecific immunolabeling of enamel protein-associated compartments by a monoclonal antibody against vimentin. J. Histochem. Cytochem. 47 1237-1245. 

M-protein (right), elucidated using immunolabeling (Obermann et al., 1997). Fast muscles have both proteins, but slow muscles have only myomesin. This correlates with the observed M-band stripes where Ml is missing in slow muscles. 

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