Immunoglobulins, crystal

These same immunoglobulin crystals have also been the subject of a study in the electron microscope. The molecule in projection appears to have a shape varying between a Y and a T, in agreement with previous electron microscope studies and the X-ray diffraction investigation. 

C Chothia, AM Lesk, M Levitt, AG Amit, RA Mariuzza, SEV Phillips, RJ Poljak. The predicted structure of immunoglobulin dl.3 and its comparison with the crystal stracture. Science 233 755-758, 1986. 

Carbohydrates, Galactomannans. antigens, interaction of, with homogeneous, murine myeloma immunoglobulins, 31, 313-346 bibliography of crystal structures of, (1967-1974), 33, 387-404 (1975), 35, 377-385 noncytotoxic, antitumor, 32, 235-275 the pneumococcal, a re-examination of, 33, 295-322... 

The crystal structure of the A-terminal domain of sialoadhesin in complex with sialyllactose as ligand has been solved at 1.85 A resolution [222]. The structure of the immunoglobulin domain contained in this receptor conforms to the V-set immunoglobulin-like fold but contains several distinctive features. The carboxylate of sialic acid forms a salt bridge with a side chain of Arg97 (O Fig. 18). 

FIGURE 1.10 Crystals of a variety of proteins. In (a) hexagonal prisms of beef liver catalase. In (b) crystals of oo acid glycoprotein, in (c) Fab fragments of a murine immunoglobulin, and in (d) rhombohedral crystals of the seed storage protein canavalin. 

FIGURE 1.12 Atomic force microscopy (AFM) also reveals the fundamental periodicity of macro-molecular crystals. In (a) is the surface layer of a crystal of brome mosaic virus, a particle having a diameter of about 280 A. In (b) is an AFM image of a monoclinic crystal of duodecahedral complexes of intact immunoglobulins, which have a diameter of about 230 A. 

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