Immunocytochemistry peroxidase method

This method, either standard or elite (increased molar ratio), remains the forefront of much of the immunocytochemistry being performed today, and will be the main subject of this chapter. There are new methods, though, that are being used with increased frequency, such as the labeled-avidin binding method, sometimes called the streptavidin-binding method, and a newer catalyzed amplification method that uses avidin, biotin, peroxidase, and a biotinyl tyramide to achieve even more sensitivity. These methods will be discussed at the end of this chapter. 

Immunocytochemistry can be a valuable tool for the determination of cellular contents from individual cell suspensions. Samples which can be analyzed include blood smears, aspirates, and swabs from any cellular site. Each sample is treated differently and yet all the methods are interchangeable. There is no one way to prepare these types of cell samples for immunocytochemical analysis. This chapter will deal with the most common forms of cell sample the swab, aspirate, smear, and touch preps. Blood can be analyzed as a smear but it presents more of a problem owing to the concentration of red blood cells. These cells have an oxidative type function and when using a peroxidase based detection system it can greatly interfere with the test. Concentrated cellular suspensions... 

---