Immunoassay effective dose

For the assay of AF exposure the use of AF-albumin adducts is a significant advance over previous methods of extrapolation from food analysis and the use of questionnaires (see A), giving a measure of biologically effective dose which may also reflect DNA damage in the liver (as discussed above). The availability of two complementary methods constitutes a strong reliable approach although it is still noteworthy that it is the immunoassay which permits the application to field studies with the hplc-fluorescence analysis limited to tens... 

Because of the possibility that the herbicide alachlor could adulterate food if either poultry or livestock consumed contaminated materials, Lehotay and Miller evaluated three commercial immunoassays in milk and urine samples from a cow dosed with alachlor. They found that milk samples needed to be diluted with appropriate solvents (1 2, v/v) to eliminate the matrix effect. One assay kit (selected based on cost) was also evaluated for use with eggs and liver samples from chickens. Egg and liver samples were blended with acetonitrile, filtered, and diluted with water. Linear calibration curves prepared from fortified egg and liver samples were identical... 

B.L. Haller, K.A. Fuller, W.S. Brown, J.W. Koenig, B J. Eveland, and M.G. Scott, Two automated prolactin immunoassays evaluated with demonstration of a high-dose hook effect in one. Clin. Chem. 38,... 

The high dose hook effect (HDH) evident as a decrease in signal with very high antigen concentration can be observed in sandwich immunoassays (in particular in single incubation assays). HDH can be prevented by diluting the samples or applying more steps in the assay. 

The total plasma concentration of lidocaine is a result of clearance of the drug and is modulated by hepatic function. There is little impact on clearance in renal disease. In situations of decreased organ perfusion, clearance is reduced and increased blood concentrations of lidocaine should be expected reduced dosing is appropriate in these circumstances. The principal binding protein of Hdocaine, AAG, has been demonstrated to accumulate after myocardial infarction. The result of accumulation of this protein is reduction of free lidocaine, which reduces the pharmacological effect of the drug. Lidocaine is usually analyzed by immunoassay, and MEGX and GX by HPLC. 

Cyclic voltammograms of working electrodes in the presence of antibodies other than anli-bioiin IgG produced no anodic peaks. For analyte solutions contain ing anti-biotin IgG, CV peak currents were proportional to the concentration of the analyie. and working dose-response curves of CV peak current versus concentration provided an effective means for completing the immunoassay. The detection limii is O.l pg/ml.. and the range of the technique is 0.1 to 1(K) pg/mL of anii-bioiin IgG. 

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