Immunoassay critical binding reagent

Immunoassays are inherently sensitive and specific. However, with continued need to develop increasingly sensitive assays to support preclinical and clinical studies, there have been ongoing efforts to enhance the capabilities of these techniques. Advances in critical binding reagents, detection systems, new assay formats and automation have resulted in improved immunoassay technology. 

Although most immunoassays have used polyclonal antibodies as the critical binding reagents, development of monoclonal antibodies by Kohler and Milstein in 1975,has resulted in their widespread use, particularly in assays for macromolecules. Their unique epitope specificity conveys advantages in double antibody immunoassays for proteins, where one monoclonal antibody may be used to capture the protein by a specific subunit or epitope, and another, directed against a... 

Immunoassays generally vary in the type of critical antibody binding reagent or the detection and reporter... 

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