Reactive oxygen species imaging

Kundu K, Knight SF, Willett N, Lee S, Taylor WR, Murthy N (2009) Hydrocyanines a class of fluorescent sensors that can image reactive oxygen species in cell culture, tissue, and in vivo. Angew Chem Int Ed 48 299-303... 

Hanson, K. M., and Clegg, R. M. 2005. Two-photon fluorescence imaging and reactive oxygen species detection within the epidermis. Methods Mol. Biol. 289 413-22. 

Recently, a ratiometric probe based on coumarin-cyanine hybrid system was selected for the study of intracellular redox processes in the absence or presence of CQ or FQ [80]. This probe was selected for its selectivity for HO radicals compared with other species of interest [81], This probe for HO radicals ratio-metric imaging displays an emission band centered at 495 nm (green) (Figure 5.7). The presence of HO elicited a marked decrease of emission intensities at 495 nm, but a significant enhancement of emission intensities at 651 nm (red). Previously, Ribaut et al. [53] have shown that erythrocytes infected by P. falciparum produce reactive oxygen species, whereas healthy erythrocytes do not However, these studies do not specify the nature of the ROS. It was suggested that some of these ROS could be HO radicals [80]. 

Gao, N., L. Li, Zh. Shi et al. 2007. High-throughput determination of glutathione and reactive oxygen species in single cells based on fluorescence images in a microchannel. Electrophoresis 28(21) 3966-3975. 

Human bladder and kidney epithelial cells were studied by SECM following an inflammatory stimulation with E. coli. Using time-lapse SECM images, the release of reactive oxygen species is linked to the presence of the toll-like receptor based on the differential response of the human bladder and kidney epithelial cells, which, respectively, express and do not express this receptor. This study is generally applicable to several critical biological processes such as cell death, lipid oxidation, and DNA damage. 

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