Hyperspectral FLIM

The fourth chapter by James McGuinty et al. describes the more advanced forms of time-domain FLIM. While not immediately available on commercial instruments this chapter should give the reader an idea what the current state-of-the-art is in terms of FLIM instrumentation, and perhaps what to expect on future commercial instruments. Real-time FLIM, combined FLIM-spectral imaging, hyperspectral FLIM-imaging, combined lifetime-anisotropy imaging and some of their applications are covered here. 

Spectral FLIM involves measuring the apparent lifetimes in a preparation at many wavelengths with the assistance of a spectrograph or a series of filters (see also Chapter 4, Figs. 4.7 and 4.8 depicting hyperspectral FLIM in the time domain). The goal of the measurement is similar to that of the multifrequency approach ... 

Fig. 4.7. Application of hyperspectral FLIM to an unstained fixed section of human artery excited at 400 nm (A) schematic of a line-scanning hyperspectral...

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