2- Hydroxyethylhydrazine, reaction with

Suicide inactivation that might be unique to EAL would be the reaction with hydroxyethylhydrazine (HEH). The complex of EAL and coenzyme reacts rapidly with HEH to produce a complex of EAL with cob(II)alamin, 5 -deoxyadenosine, and the hydrazine radical cation shown in Figure 15. Analysis of the ERR spectra for the effects of in HEH and D2O as the solvent allowed the definitive identification of the radical cation in the inactive complex. EAL in the complex could be partially reactivated by precipitation in acidic conditions, implying that the protein portions of the enzyme might be intact, with the inactivation being due to blockage of the active site by cob(II)alamin. 

P-HYDROXYETHYLHYDRAZINE (109-84-2) Combustible liquid (flash point 185°F/85°C). A reducing agent and organic base. Violent reaction with oxidizers, acids, maleic anhydride. Incompatible with organic anhydrides, acrylates, alcohols, aldehydes, alkylene oxides, substituted allyls, cellulose nitrate, cresols, caprolactam solution, 3-(3-cyano-l,2,4-oxadiazol-5-yl)-4-cyanofurazan-2-(5-) oxide, epichlorohydrin, ethylene dichloride, isocyanates, ketones, glycols, nitrates, phenols, vinyl acetate. 

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