Progesterone 17-hydroxy

Preparation of Ba-Methyi-17-Hydroxy progesterone 17-Acetate 1 g of 6a-methyl-17a-hy-droxyprogesterone was dissolved in a mixture of 10 ml of acetic acid and 2 ml of acetic anhydride by heating. After solution was effected the mixture was cooled to 15°C, and 0.3 g of p-toluenesulfonic acid was added. After allowing the mixture to stand for a period of 2 /a hours at room temperature, the pink solution was poured into ice water to give an amorphous solid which was recovered by filtration. 

The steroids aldosterone, cortisone, cortisol, 11-P-hydroxyandrostenedione, corticosterone, and rostenedione, 11-desoxycorticosterone, 17-hydroxy-progesterone, and progesterone have been performed on Ultrasphere ODS using methanokwater.19 Ranitidine N-[2-[[[5-[(dimethylamino)methyl]-2-furanyl]-methyl]thio]ethyl]-N1-methyl-2-nitro-l,l-ethenediamine has been separated using a p-Bondapak C18 column operated with acetoni-trile methanol water buffered with triethylamine phosphate.117 Pyridoxal-5 -phosphate and other B6 vitamers, including pyridoxamine phosphate, pyri-doxal, pyridoxine, and 4-pyridoxic acid, were separated as bisulfite adducts... 

P450 21A2 is the enzyme involved in the 21-hy-droxylation of progesterone and 17-hydroxy-progesterone, yielding deoxycorticosterone and 11-deoxycortisol from the two substrates, respectively (Fig. 9.12). The 21-hydroxylation reaction is an important step in the synthesis of glucocorticoids and mineralocorticoids, and de-... 

The 17-ketosteroids increased in the urine include dehydroepiandrosterone, androsterone, etiocholano-lone, and 11-hydroxy- and 11-oxy-derivatives. Thus, it would appear that as a result of the block in 21-hydroxylation, the rate of conversion of 17-hydroxy-progesterone to carbon 19 compounds is increased. These results indicated that the adrenals of these patients are capable of 11-hydroxylations in spite of the fact that 21-hydroxylation does not take place. As a result of 11-hydroxylation, 11-ketosteroids, and especially 11-ketopregnaetriol, are excreted in excessive amounts in the urine of patients with adrenogenital syndrome. Thus, 17-hydroxyprogesterone is direct-... 

This experiment (in two parts, A and B) is an introduction to the TLC separation of steroids on high-performance TLC plates. It is adapted from Whatman s TLC Technical Series, Volume 2. In A, estriol, estradiol, and estrone are separated. In B, estriol, estrone, 17-hydroxy-progesterone, pregnenolone, and progesterone are separated. 

ACTH, adrenocorticotropin hormone FSH, follicle stimulating hormone LH, luteinizing hormone 170 HP, 17-hydroxy progesterone DHEA, dehy-droepiandrosterone, A4A, androstenedione T, testosterone DHT, dihydrotestosterone E2, estradiol Aldo, aldosterone DOC, deoxycorticosterone B> corticosterone F, cortisol,... 

Fig. 22 gives a flavor of what is attainable today with a tandem mass spectrometer for the routine quantitation of aldosterone, cortisone, cortisol, 21-deoxycoitisol, corticosterone, substance S (11-deoxycortisol), 8-4-androstenedione, 21-hydroxy-progesterone, and 17-hydroxy-progesterone [27]. 

Fig. 23 shows the tracing obtained on a serum from a patient with 21-hydroxylase deficiency. A high concentration of 17-hydroxy-progesterone has been calculated at 28.9 ng/mL with the methodology. Value obtained using immunoassay was 25 ng/mL. Normal value should be less than 5 ng/mL. Peaks corresponding to substance S, cortisol, cortisone, corticosterone, and aldosterone are strongly decreased. 

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