Hydrostatic Pressing Principle

The basic principle of the application and measnranent of pressures with the FTT is shown schematically in Hgure 2.15. A capillary is inserted into a small trough of antifoam oil-in-water emnlsion and pressed against the base of the trough so that hydrostatic connection between the liqnid trapped in the capillary and that outside... 

Employing the compressibility principle whereby hydrostatic pressure selectively ejects integral membrane protein (including the putative ethylene-forming enzyme and/or ethylene receptor) from fluid phospholipid domains, isolated liquid crystalline pea foliage microsomal membranes were subjected to 1500 bar pressurization in a French Press for 20 min in the presence or absence of 10 ppm indoleacetic acid (lAA). Ethylene production of the protein-depleted membranes was compared to that of non-pressurized membranes. Ca release to the supernatant after pressurization was determined with tetramurexide. 

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