Hydrophobic hydration disappearance

In Summary, Hydrophobic Hydration Disappears for Two Different Reasons with Opposite Consequences of Insolubility and Solubility... 

Hydrophobic Hydration Increases with Increased Number of Oil-like Groups Until Solubility Is Lost and Then Hydrophobic Hydration Disappears... 

Hydrophobic Hydration Disappears When a Pair of Hydrophobic Domains Associate, That Is, When the Pair of Domains Becomes Insoluble... 

Some Hydrophobic Hydration Disappears When a Water-exposed Hydrophobic Domain Occurs Proximal to an Emergent Polar (e.g.. Charged) Species... 

The phenomenon of hydrophobic association on raising the temperature, as noted above and treated in detail in Chapter 5, derives from the thermodynamics of structured water surrounding hydrophobic moieties. Hydrophobic hydration disappears, due to an unfavorable Gibbs free energy for solubility, as the temperature is raised from below to above the transition temperature that reaehes the cusp of insolubility represented in Figure 7.1. This causes the hydrophobic domains to separate from water by means of intra- and intermolecular hydrophobic association. 

The most direct observation of changes in hydrophobic hydration attending the inverse temperature transition comes from following the temperature dependence of an absorption band in the microwave dielectric relaxation experiment shown in Figures 5.24 and 5.25. The water of hydrophobic hydration, with an absorption band near 5 GHz in the dissolved model protein, disappears on raising the temperature through the inverse temperature transition as the model protein hydrophobically... 

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