Hydrodynamic chromatography universal calibration curve

Since size exclusion chromatography separates polymer molecules by their size (especially hydrodynamic size), plotting the molecular size vs. the retention volume should be universal, regardless of the polymer molecular weight. The universal calibration curve is given as ... [Pg.440]

For determining the molar mass of branched polymers gel permeation chromatography can be used. An important quantity in this connection is the hydrodynamic volume of the polymer coil, which, as shown before in Eq. (9.27), is proportional to the product [rj M. According to Benoit and co-workers (1966) the hydrodynamic volume is the key size parameter in the establishment of a universal calibration curve for gel permeation chromatography columns (see Chap. 2) if log (h/]M) is plotted versus the elution volume for a variety of polymers, the data fit a single curve. [Pg.274]