Hydrazines, detection identification

For the practical design of hypersurfaces, i.e. cuts through the (3n-6)dimensional hyperspace, some hints are outlined. The main purpose, however, is to illustrate the usefulness of hypersurface calculations especially for the detection, identification and characterization of unstable molecules. Examples chosen comprise the structure of RS-C=C-SR, the relative stability of thioacroleine isomers C,H S, the structural changes accompanying the oxidation of hydrazine and some of its derivatives, the isomerization of tetrahedrane to cyclobutadiene both thermally as well as on oxidation, the predicted existence of F SS and nonexistence of CI2SS or H2SS, and, finally, some aspects of the thermal decomposition of methyl and vinyl azides. 

The reaction of oxyhaemoglobin with phenylhydrazine was studied in whole blood samples (Maples et al., 1988). Hydrazine-based drugs induce destruction of red blood cells with resulting haemolytic anaemia. Using DMPO as a trap, nitroxide radicals were detected, but these had solid-state or immobilized spectra with broad parallel and perpendicular features. Whilst no firm identification is possibly based on ESR spectra, except that the trapped radical must be a high polymer, various lines of evidence lead to the conclusion that the adduct was formed from a sulphydryl radical on oxyhaemoglobin. Chloroform extracts gave the phenyl radical adduct in accord with in vitro studies. 

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