Hydrazide-derivatized polystyren

Covalent linkage of IgG to hydrazide-derivatized polystyrene beads... 

Chemical Co.)- Derivatized polystyrene beads may contain hydrazide groups, alkylamine groups or Sanger s reagent (about 3 pmole reactive groups per bead) (Fig. 13.3). A typical procedure to link covalently IgG to hydrazide beads is given in Table 13.2. Beads can also be used by the method of Inman (1974) or Sanger (1945). 

Fig. 13.3. Modified polystyrene beads for covalent binding of proteins. Proteins can be linked to hydrazide beads (a) with glutaraldehyde or to beads derivatized with alkylamine using succinic anhydride and carbodiimide or Sanger s reagent (Pierce Chemical Co. Technical Information).

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