Hybridization polynucleotide kinase

T4 polynucleotide kinase (New England Biolabs, Beverly, MA, USA) phenol-chloroform (Gibco BRL, Rockville, MD, USA) 732P-AIP (NEN, Boston, MA, USA) Chroma Spin-10 columns (Clontech, Palo Alto, CA, USA) ExpressHyb Hybridization Solution (Clontech, Palo Alto, CA, USA) 20 x saline-sodium citrate (SSC) and 10% sodium dodecyl sulfate (SDS Biofluids, Rockville, MD). 

An alternative method to introducing, e.g., a polydeoxyadenylate tail onto DNA without the use of terminal deoxynucleotidyl transferase consists in 5 -phosphorylation of an appropriately tailed oligodeoxyribonucleotide, e.g. d(AATTCCCGGGAio), using T4 polynucleotide kinase, followed by annealing to d(CCCGGG) to create a hybrid containing an overlap end identical to that formed by restriction of DNA with Eco This hybrid is in turn annealed with Eco RI-... 

An oligonucleotide complementary to exon 1 sequences was labeled with [y-32p]ATP (ICN Biomedicals, CA) and T4 polynucleotide kinase (Invitrogen, Carlsbad, CA) and allowed to hybridize to sequences in 5 Xg human hippocampal poly A+ RNA. 

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