Hybrid scoring

Probe sequence, target/target hybridization [48,49], stacking interactions [40], experimental imprecision and other factors influence absolute hybridization scores. Due to these factors the full match scores for some probes can be lower than the mismatch scores of others. Because of these uncertainties, control samples or groups of sequence-related probes [12,29-32,39,50] are used to evaluate and transform absolute signals into relative values, which are then used in sequence determination. 

Keywords VS, Virtual Screening, Lead discovery, lead, HTS, Pharmacophore-Based, Structure-Based, Fragment-based, Ligand-based, Docking, Scoring, hybrid workflows, VS strategy, Benchmarking VS... 

Figure 5.2. High-throughput mating assay for two-hybrid protein interaction screening. Yeast strains containing individual bait and prey clones are combined in a well and allowed to mate. Diploids are then selected and scored for a protein-protein interaction using the selection provided by the transcriptional reporter gene.

Figure 3.3 Comparison of array CGH among DNA extracted from fresh tissue, FFPE tissue by heating protocol or nonheating protocol for two human tissue samples of metastatic carcinoma in lymph node (a-c), and undifferentiated non-small cell carcinoma (d-f). Array CGH hybridization genomic profiles show ratio values representing relative copy number of single BACs. A good result is scored as 1.0 that indicates a low standard deviation for gains (>0.2), normal (0.0), or losses (<-0.2). In these two cases, fresh samples show best score as 2, both FFPE tissue samples show identical score of 3. Each spot represents the average of three replicates. Clones are ordered by chromosomal position as numbers at the bottom (x axis) of each picture. The y axis is the log2 ratio of test reference intensity. Provided by Sandy DeVries from Dr. Frederic Waldman s Lab at UCSF.

Figure 12.31 Illustration of a hybrid calibration strategy. (A) Scatter plot of first two PCA scores obtained from a process analytical calibration data set containing both synthesized standards (circles) and actual process samples (triangles). (B) Results of a PLS regression fit to the property of Interest, using all of the calibration samples represented In (A).

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