Human serum albumin, fluorescence immunoassay

Mohamadi et al. [96] reported online preconcentration of human serum albumin (HSA) and its immunocomplex with a monoclonal antibody on-chip coupled to isotachophoresis. The sample injection, preconcentration, and separation were carried out continuously and controlled by a sequential voltage switching program. Preconcentration was carried out with on-chip nondenaturing gel electrophoresis in methylcellulose solution. Furthermore, the authors applied this method for immunoassay of HSA. The separation of HSA and its immunocomplex was achieved in 25 seconds in 1 cm of the microchannel with induced fluorescence detection at 7.5 pM. 

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