HPLC nanoscale

Comparisons of CE and HPLC on similar analytical problems have been reported [879-883]. In contrast to CE and HPLC, GC is best suited for analysis of nonpolar, lower MW, volatile compounds. HPLC and GC have detection limits roughly 100-1000 times lower than CE, while traditional electrophoresis has detection limits comparable to CE. As already mentioned, the inferior detection sensitivity and precision of CE when compared with HPLC are caused by the technique s nanoscale. 

Parker, C.E. Perkins, J.R. Tomer, K.B. Shida, Y. O Heu-a, K. Nanoscale packed capillary liquid chromatography-electrospray ionization mass spectrometry analysis of penicillins and cephems. J.Chromatogr., 1993, 616, 45-57 [capillary HPLC electrospray LC-MS serum also ampiciUin, car-benicUlin, cefalothin, cefazoUn, cefmenoxime, cefmetetzole, cefoperazone, cefotaxime, cefotiam, cefoxitin, cephalexin, cloxacillin, dicloxacillin, peniciUin G, pipericillin, sulbenicillin]... 

Nanoscale HPLC, tandem mass spectrometer Lysis... 

Note that a 100 mM Tris buffer is good to use if samples will be loaded onto a Cis enrichment desalting colunm prior to separation by nanoscale HPLC. Otherwise, use NH4HCO3. 

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