HPLC Enantioseparations using Chiral Additives to the Mobile Phase

2 HPLC Enantioseparations using Chiral Additives to the Mobile Phase 

An enantioseparation in this mode is based on the formation of non-covalent diastereomer-ic complexes between the enantiomers of an analyte and the chiral additive in the mobile phase (CAMP). Compared with indirect enantioseparations, the CAMP technique has advantages such as the absence of a derivatization step or a higher flexibility (easier change of a chiral additive than a chiral or an achiral packing material). As documented by Davan-kov [105], the enantiomer migration order with CAMP most likely will be opposite to that observed with the same chiral selector as the stationary phase. The complementary enantio-selectivity of enantioseparation with CAMP compared with CSPs is a significant advantage. 

Although some techniques are proposed for the isolation of CAMP and enantiopure analytes after a chromatographic separation [111], this is not a practicable technique for preparative purposes. 

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